Femtosecond laser induced surface modification for prevention of bacterial adhesion on 45S5 bioactive glass

摘要

Bacterial attachment and biofilm formation on implant surface has been amajor concern in hospital and industrial environment. Prevention of bacterialinfections of implant surface through surface treatment could be a potentialsolution and hence this has become a key area of research. In the presentstudy, the antibacterial and biocompatible properties of femtosecond lasersurface treated 45S5 bioactive glass (BG) have been investigated. Adhesion andsustainability of both gram positive S. aureus and gram negative P.aeruginosaand E. coli nosocomial bacteria on untreated and laser treated BG samples hasbeen explored. An imprint method has been used to visualize the growth ofbacteria on the sample surface. We observed complete bacterial rejectionpotentially reducing risk of biofilm formation on laser treated surface. Thiswas correlated with surface roughness, wettability and change in surfacechemical composition of the samples before and after laser treatment.Biocompatibility of the laser treated BG was demonstrated by studying theanchoring and growth of human cervix cell line INT407. Our results demonstratethat, laser surface modification of BG enables enhanced bacterial rejectionwithout affecting its biocompatibility towards growth of human cells on it.These results open a significantly potential approach towards use of laser insuccessfully imparting desirable characteristics to BG based bio-implants anddevices.